The cumulative permeation of FITC-dextran 10 K was 4.82 0.32 M, in the human skin and 5.92 0.46 M in the pig skin. Actually, everything depends on solvent you want to use and temperature. If you want to know gyration (Rg)or hydrodynamic (Rh) radius, you should Curley SA. 2018 Jan;184(1):1-11. doi: 10.1007/s12010-017-2522-4. where tr is the column retention time in minutes (2 of 0.9792). The dextran was chosen as another branched polysaccharide which, like the others was suspected to show hyperbranched behavior. Lpez Martnez, M. C.; Daz Baos, F. G.; Ortega Retuerta, A.; Garca de , J. Takara, Andres; Acosta, Adolfo; Masuelli, Martin A. Micropores are produced by the arrangement of microelectrodes on the skin at precise dimensions, then RF energy generated by alternating currents induces ionic vibrations between electrodes with positive or negative charges. Google Scholar. NCI CPTC Antibody Characterization Program. Experimental Review on Intrinsic Viscosity Measurements and Temperature Effect.". (1958). ; Dutt, N.V.K. Human skins (Full-thickness human skin) were supplied by HansBiomed. A single frequency laser is directed to the sample contained in a cuvette. After 24h, the cumulative permeation of FITC-dextran M.W. eCollection 2018 Feb. Molecules. Alternatives to animal testing: a review. J. Phys. CAS Please enable it to take advantage of the complete set of features! Viet, D.; Beck-Candanedo, S.; Gray D. G. (2008). Synthesis of water-soluble and water-insoluble amphiphilic derivatives of dextran in organic medium. Porcine wound models for skin substitution and burn treatment. These values indicate for dextrans in dilute aqueous solution, both methods give very similar results of the intrinsic viscosity and the respective Mw. Synthesis of dextran by dextransucrase treated, sucrose-supplemented skimmed milk revealed the promising potential of dextran as a food additive. of different origins. WebFluorescein isothiocyanatedextran average mol wt 60,000-76,000; CAS Number: 60842-46-8; Synonyms: FITCDextran; find Sigma-Aldrich-FD70S MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich (2001). Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. Manage cookies/Do not sell my data we use in the preference centre. The intrinsic viscosity can be easily calculated by the Solomon-Ciuta single-point equation [44, 45]. Charge: Anionic. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was Biomacromolecules 8, 2464-2475. Format), Citation-(BibTeX Kam Y, Sacks H, Kaplan KM, Stern M, Levin G. Radio frequency-microchannels for transdermal delivery: characterization of skin recovery and delivery window. J Pharm Sci. 2023 BioMed Central Ltd unless otherwise stated. By studying the molecular weights of various solutions of polymer, Solomon-Ciuta arrived at the formula which allowed the calculation of the intrinsic viscosity of polymer solutions by a single viscosity determination. The Dextrans intrinsic viscosity is determined as a function of the molecular weight in water and NaOH 0.5 M see reference [1]. For a rigid or rod like polymer molecule that is greatly extended in solution, the Mark-Houwink a constant approaches to a value of 2.0 [48].The hydrodynamic radius (RH), for a sphere ((a/b) = 2.5) is given by the Einstein relation [49]. 4h after microporation, the sizes of the micropore decreased slightly. These studies on Mark-Houwink parameters are usually carried out at a given temperature, obtaining a consistent result but in a very limited temperature range [68]. In addition, microporated pig skin permeated larger amounts of FITC-dextran than human skin, this can be attributed to the differences in structure between the human and pig skin; the pig skin layer is thinner (SC, 813m) than the human skin (SC, 1017m) and the pig hair follicle is longer (3871m) than the human hair follicle (18m). The above data are not useful because it can not be compared with those obtained in this work. In this work, we evaluate the maximum radius of macromolecules able to enter into HeLa cells following a plasma treatment using a size exclusion approach with dextran molecules. Kloster, C.; Bica C.; Rochas C.; Samios, D.; Geissler, E. (2000). European Biophysical Journal, 36, 85-94. The characteristic Rg/Rh value for a globular protein is ~0.775, which means that Rg is smaller than Rh. Pig skins (Micropig Franz cell membrane) were supplied by Medi Kinetics Co., Ltd. (Pyeongtaek, Korea). WebFluorescent and biotinylated dextrans are routinely employed to trace neuronal projections. Figure1 shows a schematic illustration of transdermal delivery after the application of the microporation device. Chapter 2.1.4 Suspended level viscometers for transparent liquid, Ubbelohde Viscometer page 28-31. Salunkhe SS, Bhatia NM, Pokharkar VB, Thorat JD, Bhatia MS. Topical delivery of idebenone using nanostructured lipid carriers: evaluations of sun-protection and anti-oxidant effects. ; Ghosh, T.K. Dextran is a branched polysaccharide, composed of -D-glucopyranosyl residues. For 40 kDa molecules, the hydrodynamic diameter d h ~8 nm is much smaller than the collagen mesh size [33,41]. (1937). The regeneration of micropores in the human cadaver skin caused by microporation was confirmed using an optical microscope and haematoxylin/eosin (H&E) staining. Pribush, A.; Zilberman-Kravits, D.; Meyerstein, N. (2007). A novel high dextran yielding Weissella cibaria JAG8 for cereal food application. 2022 Feb 22;16(2):1999-2012. doi: 10.1021/acsnano.1c07010. Biotechnology Progress, 19, 544-548. The solution viscosity () is related to the solvent viscosity (o), resulting in the relative viscosity. ; De Jong, S.J. De Belder, A.N. The parameters of Mark-Houwink for polymers can vary with the polymer solution and temperature. Theory, Estimation, Experiment, and Data. 4K, 10K, and 20K) permeated through the human, and pig skin after microporation. 2004;25(9):155967. We offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges. Food Sci Biotechnol. Data File Dextran. Formul. (2003). The optical image and histological analysis confirmed that the micropores on the skin were recovered over time. Bohdanecky, Miloslav (1996). 2021 Aug;13(4):915-925. doi: 10.1007/s12602-021-09751-1. Biological skin substitutes for wound cover and closure. Hi Sir Alexander Malm , Thank you very much! Really appreciate your help! Levin G, Gershonowitz A, Sacks H, Stern M, Sherman A, Rudaev S, Zivin I, Phillip M. Transdermal delivery of human growth hormone through RF-microchannels. These results suggest that fibroblasts proliferate and migrate under the SC, and regenerate the skin. Teixeira CG, Fusieger A, Milio GL, Martins E, Drider D, Nero LA, de Carvalho AF. Ind. Cytotoxicity studies on human cervical cancer (HeLa) cell line showed non-toxic and biocompatible nature of dextran. Expert Opin Drug Deliv. Banga AK. In the range of working temperatures and molecular weights of dextrans, similar intrinsic viscosity results obtained by other researchers and published here are slight, as shown in tables 1 and 3. (2007). Google Scholar. Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextransucrase. Pharm Res. Pamis, R.; Hernndez Cifre, J. G.; Lpez Martnez, M.C. For a sphere and a random coil 0 the values are 9.23x1023 mol-1 and 2.60x 1023mol-1, respectively. (2008). Figure 3 shows that the parameters vary with temperature, evidencing a clear functionality of them. Carbohydrate Polymers, 38, 267-271. Determination of polymer structure by Gel Permeation Chromatography. The same way, many of these authors emphasize the non-linear or hyperbranched of dextran molecular weight range from 9 to 2000kDa [3,5,8,16, 37,38]. Terms and Conditions, This device can generate microchannels from the SC through to the outer dermis and allow transdermal delivery of active agents. The equation 4 can be rearranged and resulted in a modified M-H equation as follows: where the value qM-H is the polydispersity correction factor, and it is a statistical function of the molecular weight distribution. Home > 2022 > Junho > 22 > Uncategorized > dextran hydrodynamic radius. National Library of Medicine The Mark-Houwink. Harding, S. E.; Varum, K.; Stoke B.; Smidsrod, O. The relation between Mw and the intrinsic viscosity is given by Mark-Houwink equation. This universalization of the parameters leads to a higher error ratio in the calculation of the respective molecular weights. 1994, 95-0355. WebThomas Gutberlet works at Jlich Centre for Neutron Science (JCNS-2), Forsch and is well known for Lipoteichoic Acid, Neutron Reflectometry and Neutron Reflectivity. Our portfolio includes for example derivatives of dextran, polysucrose, inulin and more. Expert Rev Med Devices. Carbohydr Polym. The site is secure. Eremeeva, T. E.; Bykova, T. O. In contrast to linear chains, power law behavior is observed only asymptotically at large Mv. 24h after microporation, the pore sizes reduced from 64.8817.20m (0h) to 48.854.27m (24h), and the pore depths reduced from 83.9120.56m (0h) to 63.9527.61m (24h). 2003;89(2):31120. Barrier integrity was examined using Tewameter to confirm the poration of human cadaver skin using a microporation device. The mechanism of the dextran-induced red blood cell Aggregation. Recent developments in polysaccharide-based materials used as adsorbents in wastewater treatment. Red blood cell rouleaux formation in dextran solution: dependence on polymer conformation. For Research Use Only. Differences were considered statistically significant at p <0.05. In the future, we plan to investigate the possibility of enhancing the delivery of other macromolecular drugs such as peptides and siRNAs using the RF-based microporation device. We believe that the microporation device can be used as a potential technique for effective transdermal drug delivery. Fixable: Fixable via Lysine. Typical examples are the classical size-independent combinations like the Flory parameter, which that combine the intrinsic viscosity, [], and the gyration radius, Rg: These quantities have been proposed along the years, by different eminent scientists, after whom they are named. Hydrodynamic Properties of Tragacanthin. Carbohydrate Polymers, 79, 380-390. FITC-dextran solution was prepared at 1mM in PBS. 2018;1:10915. Superior prebiotic and physicochemical properties of novel dextran from Weissella cibaria JAG8 for potential food applications. Cookies policy. Normally, the skin loses very little water loss whereas damaged skin loses more [22]. To confirm the extent of skin damage and regeneration after microporation, porations were performed 2, 5, and 10 times. WebDextran 670 GPC Standard Normative Mw - 670000 Da GPC standards can be used for calibration and molecular weight determination. Keratinocytes adjacent to the wound site induced by the microporation in the epidermis undergo a series of modifications that allow their proliferation and migration to the wound. Monosaccharide analysis revealed that the polysaccharide comprised only glucose units. For rigid macromolecules, it is also conventional to combine a solution property with the volume of the particle itself, or with a quantity directly derived from it [54, 55, 56]. The dextran has the advantage in terms of low cytotoxicity, excellent biocompatibility, and water-solubility. Drug deliv. The human and pig skin permeability of FITC-dextran (M.W. The lack of data on the uniformity of intrinsic viscosity measurements in the system water/dextran, highlights the significant influence of the solvent and temperature [74]. The calculated dextran molecular weights are corroborated by those tabulated data by the manufacturer with a relative error lower than 10%, being the exception dextrans 580 and 2000kDa with the highest relative error. ; Aberle, T; Burchard, W. (2000). He, L.; Niemeyer, B. ; Yevlampieva N.P. In a poor solvent which shows a positive heat of mixing, segments of a polymer molecule attract each other in solution more strongly than attract the surrounding solvent molecules. The importance of this type of study lies in the analysis of the polysaccharide behavior at industrial processes, the requirements to reduce energy, avoid flow problems and product quality control. In the case of the work performed in references [34, 35, 36] they studied using the number average molecular weight, which rearranges the value of a and forces it to enter into unbranched or linear molecules, on the other hand, if molecular weights are used the value would be less than 0.5. Determination of constants, kM-H and a, from the intrinsic viscosity data, requires a series of monodisperse polymer samples with known molecular weight or a series of polydisperse polymer samples with known viscosity average molecular weights, Mv.
dextran hydrodynamic radius
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